Optimum conditions for selective isolation of genes from complex genomes by transformation-associated recombination cloning

Transformation-associated recombination (TAR) cloning in yeast is used to isolate a desired chromosomal region or gene from a complex genome without construction of a genomic library. The technique involves homologous recombination during yeast spheroplast transformation between genomic DNA and a TA...

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Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Leem, Sun-Hee, Noskov, Vladimir N., Park, Jung-Eun, Kim, Seung Il, Larionov, Vladimir, Kouprina, Natalay
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 2003
Témata:	NAR Methods Online
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC152883/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12626728/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng029
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