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Construction and purification of site-specifically modified DNA templates for transcription assays

Chemical and physical agents can alter the structure of DNA by modifying the bases and the phosphate–sugar backbone, consequently compromising both replication and transcription. During transcription elongation, RNA polymerase complexes can stall at a damaged site in DNA and mark the lesion for repa...

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Detalles Bibliográficos
Main Authors: Perlow, Rebecca A., Schinecker, Thomas M., Kim, Se Jun, Geacintov, Nicholas E., Scicchitano, David A.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2003
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC152825/
https://ncbi.nlm.nih.gov/pubmed/12655028
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