Conditional gene expression by controlling translation with tetracycline-binding aptamers

We present a conditional gene expression system in Saccharomyces cerevisiae which exploits direct RNA–metabolite interactions as a mechanism of genetic control. We inserted preselected tetracycline (tc) binding aptamers into the 5′-UTR of a GFP encoding mRNA. While aptamer insertion generally reduce...

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Autores principales: Suess, Beatrix, Hanson, Shane, Berens, Christian, Fink, Barbara, Schroeder, Renée, Hillen, Wolfgang
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2003
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Articles
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC152801/
https://ncbi.nlm.nih.gov/pubmed/12655001
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