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A yeast-based growth assay for the analysis of site-specific proteases

Many cellular and viral processes depend on site-specific proteolysis. Here, a genetic system for the identification of such proteases and inhibitors is described. The system utilizes the temperature- sensitive Saccharomyces cerevisiae CDC25-2 mutant strain and exploits the strict requirement of mem...

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Autor principal: Köhler, Fabian
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2003
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC150248/
https://ncbi.nlm.nih.gov/pubmed/12582261
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