Controlling gene expression in yeast by inducible site-specific recombination

An intron module was developed for Saccharomyces cerevisiae that imparts conditional gene regulation. The kanMX marker, flanked by loxP sites for the Cre recombinase, was embedded within the ACT1 intron and the resulting module was targeted to specific genes by PCR-mediated gene disruption. Initiall...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Cheng, Tzu-Hao, Chang, Chuang-Rung, Joy, Prabha, Yablok, Svetlana, Gartenberg, Marc R.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2000
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC115252/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11121495/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.24.e108
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