Controlling gene expression in yeast by inducible site-specific recombination

An intron module was developed for Saccharomyces cerevisiae that imparts conditional gene regulation. The kanMX marker, flanked by loxP sites for the Cre recombinase, was embedded within the ACT1 intron and the resulting module was targeted to specific genes by PCR-mediated gene disruption. Initiall...

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Publicado en:	Nucleic Acids Res
Autores principales:	Cheng, Tzu-Hao, Chang, Chuang-Rung, Joy, Prabha, Yablok, Svetlana, Gartenberg, Marc R.
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Oxford University Press 2000
Materias:	NAR Methods Online
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC115252/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11121495/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.24.e108
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