In vitro transposition of Tn552: a tool for DNA sequencing and mutagenesis.

We have explored the potential of the Tn 552 in vitro transposition reaction as a genetic tool. The reaction is simple (requiring a single protein component), robust and efficient, readily producing insertions into several percent of target DNA. Most importantly, Tn 552 insertions in vitro appear to...

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Detalles Bibliográficos
Autores principales: Griffin, T J, Parsons, L, Leschziner, A E, DeVost, J, Derbyshire, K M, Grindley, N D
Formato: Artigo
Lenguaje:Inglês
Publicado: 1999
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC148649/
https://ncbi.nlm.nih.gov/pubmed/10481025
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