In vitro transposition of Tn552: a tool for DNA sequencing and mutagenesis.

We have explored the potential of the Tn 552 in vitro transposition reaction as a genetic tool. The reaction is simple (requiring a single protein component), robust and efficient, readily producing insertions into several percent of target DNA. Most importantly, Tn 552 insertions in vitro appear to...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Griffin, T J, Parsons, L, Leschziner, A E, DeVost, J, Derbyshire, K M, Grindley, N D
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1999
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148649/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10481025/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.19.3859
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