In vitro transposition of Tn552: a tool for DNA sequencing and mutagenesis.
We have explored the potential of the Tn 552 in vitro transposition reaction as a genetic tool. The reaction is simple (requiring a single protein component), robust and efficient, readily producing insertions into several percent of target DNA. Most importantly, Tn 552 insertions in vitro appear to...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1999
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148649/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10481025/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.19.3859 |
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