In vitro transposition of Tn552: a tool for DNA sequencing and mutagenesis.

We have explored the potential of the Tn 552 in vitro transposition reaction as a genetic tool. The reaction is simple (requiring a single protein component), robust and efficient, readily producing insertions into several percent of target DNA. Most importantly, Tn 552 insertions in vitro appear to...

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Bibliografiske detaljer
Main Authors: Griffin, T J, Parsons, L, Leschziner, A E, DeVost, J, Derbyshire, K M, Grindley, N D
Format: Artigo
Sprog:Inglês
Udgivet: 1999
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC148649/
https://ncbi.nlm.nih.gov/pubmed/10481025
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