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Crosslinking of proteins to DNA in human nuclei using a 60 femtosecond 266 nm laser.

We developed appropriate conditions to use a laser with 60 femtosecond pulses, a frequency of 1 KHz and a wavelength of 266 nm to efficiently crosslink proteins to DNA in human nuclei for the purpose of using immunoprecipitation to study the binding of specific proteins to specific sequences of DNA...

Cur síos iomlán

Sábháilte in:
Sonraí bibleagrafaíochta
Foilsithe in:Nucleic Acids Res
Príomhchruthaitheoirí: Lejnine, S, Durfee, G, Murnane, M, Kapteyn, H C, Makarov, V L, Langmore, J P
Formáid: Artigo
Teanga:Inglês
Foilsithe / Cruthaithe: Oxford University Press 1999
Ábhair:
Rochtain ar líne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148622/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10471736/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.18.3676
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