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Site-directed psoralen crosslinking of DNA.

A technique has been developed for placing site-specific crosslinks into DNA by using a psoralen derivative containing a sulfhydryl group. Plasmid pBR322 was nicked at the unique BamHI restriction site, and mercurated pyrimidines were introduced by nick-translation. Then the psoralen was specificall...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Saffran, W A, Goldenberg, M, Cantor, C R
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1982
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC346721/
https://ncbi.nlm.nih.gov/pubmed/6289307
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