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Crosslinking of proteins to DNA in human nuclei using a 60 femtosecond 266 nm laser.

We developed appropriate conditions to use a laser with 60 femtosecond pulses, a frequency of 1 KHz and a wavelength of 266 nm to efficiently crosslink proteins to DNA in human nuclei for the purpose of using immunoprecipitation to study the binding of specific proteins to specific sequences of DNA...

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Udgivet i:Nucleic Acids Res
Principais autores: Lejnine, S, Durfee, G, Murnane, M, Kapteyn, H C, Makarov, V L, Langmore, J P
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1999
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148622/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10471736/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.18.3676
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