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Linkers designed to intercalate the double helix greatly facilitate DNA alkylation by triplex-forming oligonucleotides carrying a cyclopropapyrroloindole reactive moiety.

Triplex-forming oligonucleotides (TFOs) bind sequence-specifically in the major groove of double-stranded DNA. Cyclopropapyrroloindole (CPI), the electrophilic moiety that comprises the reactive subunit of the antibiotic CC-1065, gives hybridization-triggered alkylation at the N-3 position of adenin...

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Udgivet i:Nucleic Acids Res
Principais autores: Dempcy, R O, Kutyavin, I V, Mills, A G, Lukhtanov, E A, Meyer, R B
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1999
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148509/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10390536/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.14.2931
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