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Chemical rescue, multiple ionizable groups, and general acid–base catalysis in the HDV genomic ribozyme

In the ribozyme from the hepatitis delta virus (HDV) genomic strand RNA, a cytosine side chain is proposed to facilitate proton transfer in the transition state of the reaction and, thus, act as a general acid–base catalyst. Mutation of this active-site cytosine (C75) reduced RNA cleavage rates by a...

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Detalhes bibliográficos
Main Authors: Perrotta, Anne T., Wadkins, Timothy S., Been, Michael D.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1484427/
https://ncbi.nlm.nih.gov/pubmed/16690998
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.14106
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