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Simultaneous, Coincident Optical Trapping and Single-Molecule Fluorescence

We constructed a microscope-based instrument capable of simultaneous, spatially coincident optical trapping and single-molecule fluorescence. The capabilities of this apparatus were demonstrated by studying the force-induced strand separation of a dye-labeled, 15-basepair region of double stranded D...

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Detalhes bibliográficos
Main Authors: Lang, Matthew J., Fordyce, Polly M., Engh, Anita M., Neuman, Keir C., Block, Steven M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1483847/
https://ncbi.nlm.nih.gov/pubmed/15782176
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth714
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