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Measurement of the effective focal shift in an optical trap
The focus of an oil-immersion microscope objective is shifted because of the refractive-index mismatch between the cover glass and the aqueous sample. We present a procedure with which to determine the focal shift by use of an inverted microscope equipped with optical tweezers. As the position of th...
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| Huvudupphovsmän: | , , |
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| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
2005
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1483143/ https://ncbi.nlm.nih.gov/pubmed/15981519 |
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