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Measurement of the effective focal shift in an optical trap

The focus of an oil-immersion microscope objective is shifted because of the refractive-index mismatch between the cover glass and the aqueous sample. We present a procedure with which to determine the focal shift by use of an inverted microscope equipped with optical tweezers. As the position of th...

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Autors principals: Neuman, Keir C., Abbondanzieri, Elio A., Block, Steven M.
Format: Artigo
Idioma:Inglês
Publicat: 2005
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1483143/
https://ncbi.nlm.nih.gov/pubmed/15981519
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