Low temperature cycled PCR protocol for Klenow fragment of DNA polymerase I in the presence of proline.
A method for performing cycled PCR at low temperatures, using the thermolabile Klenow fragment of DNA polymerase I, is reported. Application of proline as a buffer additive in the range of 3.0-5.5 M remarkably increases the thermal stability of the polymerase and decreases the denaturation temperatu...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1999
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148357/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10037825/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.6.1566 |
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