Low temperature cycled PCR protocol for Klenow fragment of DNA polymerase I in the presence of proline.

A method for performing cycled PCR at low temperatures, using the thermolabile Klenow fragment of DNA polymerase I, is reported. Application of proline as a buffer additive in the range of 3.0-5.5 M remarkably increases the thermal stability of the polymerase and decreases the denaturation temperatu...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Iakobashvili, R, Lapidot, A
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1999
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148357/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10037825/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.6.1566
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