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Mapping epitopes and antigenicity by site-directed masking
Here we describe a method for mapping the binding of antibodies to the surface of a folded antigen. We first created a panel of mutant antigens (β-lactamase) in which single surface-exposed residues were mutated to cysteine. We then chemically tethered the cysteine residues to a solid phase, thereby...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
2006
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1482585/ https://ncbi.nlm.nih.gov/pubmed/16754878 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0600263103 |
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