A method of one-step enzyme labelling of short oligonucleotide probes for filter hybridisation.
Here we describe a method of labelling short oligonucleotide probes with enzyme without purification or chemical modifications. Biotinylated oligonucleotides as short as 10 nt are coupled with streptavidin-conjugated enzyme, hybridised and detected with enzyme-triggered chemiluminescence. The detect...
保存先:
| 出版年: | Nucleic Acids Res |
|---|---|
| 主要な著者: | , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1999
|
| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148235/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9863000/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.2.703 |
| タグ: |
タグなし, このレコードへの初めてのタグを付けませんか!
|