Identification of individual prokaryotic cells by using enzyme-labeled, rRNA-targeted oligonucleotide probes.

A method to microscopically detect and identify individual cells of members of the domains Bacteria and Archaea is presented. rRNA-targeted oligonucleotides were 5' end labeled with the enzyme horseradish peroxidase and used for whole-cell hybridization. Specifically bound probe was visualized by th...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Amann, R I, Zarda, B, Stahl, D A, Schleifer, K H
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1992
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183040/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1444414/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.9.3007-3011.1992
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