A method of one-step enzyme labelling of short oligonucleotide probes for filter hybridisation.

Here we describe a method of labelling short oligonucleotide probes with enzyme without purification or chemical modifications. Biotinylated oligonucleotides as short as 10 nt are coupled with streptavidin-conjugated enzyme, hybridised and detected with enzyme-triggered chemiluminescence. The detect...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Guerasimova, A, Ivanov, I, Lehrach, H
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1999
Gaiak:
Research Article
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC148235/
https://ncbi.nlm.nih.gov/pubmed/9863000
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