A method of one-step enzyme labelling of short oligonucleotide probes for filter hybridisation.
Here we describe a method of labelling short oligonucleotide probes with enzyme without purification or chemical modifications. Biotinylated oligonucleotides as short as 10 nt are coupled with streptavidin-conjugated enzyme, hybridised and detected with enzyme-triggered chemiluminescence. The detect...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1999
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148235/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9863000/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.2.703 |
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