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Steric factors influencing hybridisation of nucleic acids to oligonucleotide arrays.

We have investigated the use of spacer molecules to reduce steric interference of the support on the hybridisation behaviour of immobilised oligonucleotides. These spacers are built up from a variety of monomeric units, using phosphoramidite chemistry, by condensation onto an amine-functionalised po...

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Библиографические подробности
Главные авторы: Shchepinov, M S, Case-Green, S C, Southern, E M
Формат: Artigo
Язык:Inglês
Опубликовано: 1997
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC146580/
https://ncbi.nlm.nih.gov/pubmed/9092624
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