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Topoisomerase II, not topoisomerase I, is the proficient relaxase of nucleosomal DNA

Eukaryotic topoisomerases I and II efficiently remove helical tension in naked DNA molecules. However, this activity has not been examined in nucleosomal DNA, their natural substrate. Here, we obtained yeast minichromosomes holding DNA under (+) helical tension, and incubated them with topoisomerase...

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Detalhes bibliográficos
Main Authors: Salceda, Javier, Fernández, Xavier, Roca, Joaquim
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1478187/
https://ncbi.nlm.nih.gov/pubmed/16710299
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.emboj.7601142
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