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Combination of Multiplex PCR and PCR-Denaturing Gradient Gel Electrophoresis for Monitoring Common Sourdough-Associated Lactobacillus Species
A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtain...
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Main Authors: | , , , , , |
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Format: | Artigo |
Jezik: | Inglês |
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American Society for Microbiology
2006
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Teme: | |
Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1472401/ https://ncbi.nlm.nih.gov/pubmed/16672538 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.5.3793-3796.2006 |
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