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Combination of Multiplex PCR and PCR-Denaturing Gradient Gel Electrophoresis for Monitoring Common Sourdough-Associated Lactobacillus Species

A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtain...

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Autors principals: Settanni, Luca, Valmorri, Sara, van Sinderen, Douwe, Suzzi, Giovanna, Paparella, Antonello, Corsetti, Aldo
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2006
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1472401/
https://ncbi.nlm.nih.gov/pubmed/16672538
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.5.3793-3796.2006
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