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Combination of Multiplex PCR and PCR-Denaturing Gradient Gel Electrophoresis for Monitoring Common Sourdough-Associated Lactobacillus Species

A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtain...

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Detalhes bibliográficos
Main Authors: Settanni, Luca, Valmorri, Sara, van Sinderen, Douwe, Suzzi, Giovanna, Paparella, Antonello, Corsetti, Aldo
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1472401/
https://ncbi.nlm.nih.gov/pubmed/16672538
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.5.3793-3796.2006
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