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Improvement of an Unusual Twin-Arginine Transporter Leader Peptide by a Codon-Based Randomization Approach
Secretion of Escherichia coli penicillin acylase was improved by codon-based random mutagenesis of its signal peptide. The mutagenesis technology was applied to the gene region coding for positions Lys2 to Thr13 (N half) and Ala14 to Leu25 (C half) of the signal peptide. Protein secretion was higher...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology
2006
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1472356/ https://ncbi.nlm.nih.gov/pubmed/16672539 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.5.3797-3801.2006 |
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