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Novel ceftazidime-resistance β-lactamases generated by a codon-based mutagenesis method and selection

Four known and nine new ceftazidime-resistance β-lactamases were generated by a novel, contaminating codon-based mutagenesis approach. In this method, wild-type codons are spiked with a set of mutant codons during oligonucleotide synthesis, generating random combinatorial libraries of primers that c...

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Détails bibliographiques
Auteurs principaux: Gaytán, Paul, Osuna, Joel, Soberón, Xavier
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2002
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC134257/
https://ncbi.nlm.nih.gov/pubmed/12177312
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