Conformational Heterogeneity in RNA Polymerase Observed by Single-Pair FRET Microscopy

Kinetic, structural, and single-molecule transcription measurements suggest that RNA polymerase can adopt many different conformations during elongation. We have measured the geometry of the DNA and RNA in ternary elongation complexes using single-pair fluorescence resonance energy transfer. Six dif...

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Detalhes bibliográficos
Main Authors: Coban, Oana, Lamb, Don C., Zaychikov, Evgeny, Heumann, Hermann, Nienhaus, G. Ulrich
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2006
Assuntos:
Proteins
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1471840/
https://ncbi.nlm.nih.gov/pubmed/16581837
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.078840
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