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Real-time characterization of intermediates in the pathway to open complex formation by Escherichia coli RNA polymerase at the T7A1 promoter

We have used time-resolved x-ray-generated hydroxyl radical footprinting to directly characterize, at single-nucleotide resolution, several intermediates in the pathway to open complex formation by Escherichia coli RNA polymerase on the T7A1 promoter at 37°C. Three sets of intermediates, correspondi...

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書誌詳細
主要な著者: Sclavi, Bianca, Zaychikov, Evgeny, Rogozina, Anastasia, Walther, Ferdinand, Buckle, Malcolm, Heumann, Hermann
フォーマット: Artigo
言語:Inglês
出版事項: National Academy of Sciences 2005
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オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC555702/
https://ncbi.nlm.nih.gov/pubmed/15738402
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0408218102
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