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Avoidance of sulfur loss during ammonia treatment of oligonucleotide phosphorothioates.

Sulfur loss during the unblocking of phosphorothioate analogues of oligonucleotides with concentrated aqueous ammonia can be completely suppressed by the addition of 2-mercaptoethanol.

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Bibliografiska uppgifter
I publikationen:Nucleic Acids Res
Huvudupphov: Reese, C B, Song, Q
Materialtyp: Artigo
Språk:Inglês
Utgiven: Oxford University Press 1997
Ämnen:
Länkar:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146813/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9207048/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.14.2943
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