Avoidance of sulfur loss during ammonia treatment of oligonucleotide phosphorothioates.
Sulfur loss during the unblocking of phosphorothioate analogues of oligonucleotides with concentrated aqueous ammonia can be completely suppressed by the addition of 2-mercaptoethanol.
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1997
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146813/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9207048/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.14.2943 |
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