Avoidance of sulfur loss during ammonia treatment of oligonucleotide phosphorothioates.
Sulfur loss during the unblocking of phosphorothioate analogues of oligonucleotides with concentrated aqueous ammonia can be completely suppressed by the addition of 2-mercaptoethanol.
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| Publicado en: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1997
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146813/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9207048/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.14.2943 |
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