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Efficient priming of PCR with short oligonucleotides conjugated to a minor groove binder.

The tripeptide 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI3) binds to the minor groove of DNA with high affinity. When this minor groove binder (MGB) is conjugated to the 5'-end of short oligodeoxynucleotides (ODNs), the conjugates form unusually stable hybrids with complementary DNA i...

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Udgivet i:Nucleic Acids Res
Principais autores: Afonina, I, Zivarts, M, Kutyavin, I, Lukhtanov, E, Gamper, H, Meyer, R B
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1997
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146788/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9185578/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.13.2657
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