Efficient priming of PCR with short oligonucleotides conjugated to a minor groove binder.
The tripeptide 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI3) binds to the minor groove of DNA with high affinity. When this minor groove binder (MGB) is conjugated to the 5'-end of short oligodeoxynucleotides (ODNs), the conjugates form unusually stable hybrids with complementary DNA i...
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| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1997
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| 主題: | |
| オンライン・アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146788/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9185578/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.13.2657 |
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