Rapid selection of aminoacyl-tRNAs based on biotinylation of alpha-NH2 group of charged amino acids.

A rapid selection procedure to separate low amounts of aminoacylated tRNAs from large pools of inactive variants is described. The procedure involves a three-step protocol. After initial aminoacylation of a tRNA pool, N-hydroxysuccinimide ester chemistry is applied to biotinylate the alpha-NH2 group...

Descripció completa

Dades bibliogràfiques

Publicat a:	Nucleic Acids Res
Autors principals:	Pütz, J, Wientges, J, Sissler, M, Giegé, R, Florentz, C, Schwienhorst, A
Format:	Artigo
Idioma:	Inglês
Publicat:	Oxford University Press 1997
Matèries:	Research Article
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146665/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9162902/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.9.1862
Etiquetes:	Afegir etiqueta Sense etiquetes, Sigues el primer a etiquetar aquest registre!