Rapid selection of aminoacyl-tRNAs based on biotinylation of alpha-NH2 group of charged amino acids.
A rapid selection procedure to separate low amounts of aminoacylated tRNAs from large pools of inactive variants is described. The procedure involves a three-step protocol. After initial aminoacylation of a tRNA pool, N-hydroxysuccinimide ester chemistry is applied to biotinylate the alpha-NH2 group...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1997
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146665/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9162902/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.9.1862 |
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