Rapid selection of aminoacyl-tRNAs based on biotinylation of alpha-NH2 group of charged amino acids.

A rapid selection procedure to separate low amounts of aminoacylated tRNAs from large pools of inactive variants is described. The procedure involves a three-step protocol. After initial aminoacylation of a tRNA pool, N-hydroxysuccinimide ester chemistry is applied to biotinylate the alpha-NH2 group...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Pütz, J, Wientges, J, Sissler, M, Giegé, R, Florentz, C, Schwienhorst, A
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 1997
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146665/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9162902/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.9.1862
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