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Ligation-mediated PCR amplification of specific fragments from a class-II restriction endonuclease total digest.

A method is described which permits the ligation- mediated PCR amplification of specific fragments from a Class-II restriction endonuclease total digest. Feasibility was tested using Bcl I and phage lambda DNA as a model enzyme and amplicon system, respectively. Bcl I is one of many widely used rest...

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Bibliografische gegevens
Hoofdauteurs: Guilfoyle, R A, Leeck, C L, Kroening, K D, Smith, L M, Guo, Z
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1997
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC146646/
https://ncbi.nlm.nih.gov/pubmed/9108171
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