Ligation-mediated PCR amplification of specific fragments from a class-II restriction endonuclease total digest.

A method is described which permits the ligation- mediated PCR amplification of specific fragments from a Class-II restriction endonuclease total digest. Feasibility was tested using Bcl I and phage lambda DNA as a model enzyme and amplicon system, respectively. Bcl I is one of many widely used rest...

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Bibliographic Details

Published in:	Nucleic Acids Res
Main Authors:	Guilfoyle, R A, Leeck, C L, Kroening, K D, Smith, L M, Guo, Z
Format:	Artigo
Language:	Inglês
Published:	Oxford University Press 1997
Subjects:	Research Article
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146646/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9108171/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.9.1854
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