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Ligation-mediated PCR amplification of specific fragments from a class-II restriction endonuclease total digest.

A method is described which permits the ligation- mediated PCR amplification of specific fragments from a Class-II restriction endonuclease total digest. Feasibility was tested using Bcl I and phage lambda DNA as a model enzyme and amplicon system, respectively. Bcl I is one of many widely used rest...

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Bibliografiska uppgifter
I publikationen:Nucleic Acids Res
Huvudupphov: Guilfoyle, R A, Leeck, C L, Kroening, K D, Smith, L M, Guo, Z
Materialtyp: Artigo
Språk:Inglês
Utgiven: Oxford University Press 1997
Ämnen:
Länkar:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146646/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9108171/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.9.1854
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