Ligation-mediated PCR amplification of specific fragments from a class-II restriction endonuclease total digest.

A method is described which permits the ligation- mediated PCR amplification of specific fragments from a Class-II restriction endonuclease total digest. Feasibility was tested using Bcl I and phage lambda DNA as a model enzyme and amplicon system, respectively. Bcl I is one of many widely used rest...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Guilfoyle, R A, Leeck, C L, Kroening, K D, Smith, L M, Guo, Z
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 1997
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146646/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9108171/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.9.1854
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