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A new, rapid and simple procedure for direct cloning of PCR products into baculoviruses.

We propose a novel method for direct cloning of foreign genes into baculoviruses which avoids the use of bacterial transfer vectors. The foreign gene to be inserted is derived by PCR using appropriate primers each of which contains an additional 50 nt of baculovirus sequence for homologous recombina...

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Udgivet i:Nucleic Acids Res
Principais autores: Gritsun, T S, Mikhailov, M V, Roy, P, Gould, E A
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1997
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146644/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9108173/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.9.1864
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