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A positive screen for cloning PCR products.

We have developed a positive screen for cloning PCR products based on translational activation of lacZ. A vector with a translationally deficient lacZ alpha gene has been made by deletion of the Shine-Dalgarno sequence and initiation codon. The Shine-Dalgarno sequence and initiation codon are incorp...

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Sonraí Bibleagrafaíochta
Main Authors: Keese, P, Graf, L
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1996
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC146101/
https://ncbi.nlm.nih.gov/pubmed/8811107
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