A positive screen for cloning PCR products.
We have developed a positive screen for cloning PCR products based on translational activation of lacZ. A vector with a translationally deficient lacZ alpha gene has been made by deletion of the Shine-Dalgarno sequence and initiation codon. The Shine-Dalgarno sequence and initiation codon are incorp...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1996
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146101/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8811107/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.17.3474 |
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