A new, rapid and simple procedure for direct cloning of PCR products into baculoviruses.

We propose a novel method for direct cloning of foreign genes into baculoviruses which avoids the use of bacterial transfer vectors. The foreign gene to be inserted is derived by PCR using appropriate primers each of which contains an additional 50 nt of baculovirus sequence for homologous recombina...

Полное описание

Библиографические подробности

Опубликовано в::	Nucleic Acids Res
Главные авторы:	Gritsun, T S, Mikhailov, M V, Roy, P, Gould, E A
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Oxford University Press 1997
Предметы:	Research Article
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146644/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9108173/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.9.1864
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